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感染症基因體中心Genome Center for Infectious Diseases
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:::Genomic Center for Infectious Diseases > Core Technologies

Core Technologies

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Updated 2023/5/1 9:35:57
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Each infectious pathogen has its own special genetic composition. The center decodes the genetic composition of the pathogen through metagenomics next-generation sequencing (mNGS) and compares the sequenced genome with the reference sequences in the database. A single test can identify multiple pathogens in the sample.
Sample collection Sample collection
Collecting sample from infected paitnets. Sample types include blood, alveolar lavage fluid, tissue, etc.
DNA extration & Library preparation DNA extration & Library preparation
The DNA/RNA in the sample is extracted through a validated extraction method, and optimized for the type of sample to prepare a sample library that can be used for sequencing. There is quality control throughout the process.
Sequencing Sequencing
Using Shotgun next-generation sequencing technology to detect all genetic components in the sample and obtain sequence data.
Through sequencing of a large number of short sequence fragments simultaneously, a high-throughput gene decoding is achieved. 
Analysis Analysis
Using the unique pathogen database (including pathogen sequence data of tens of thousands of cross-species), compare and analyze the sequenced data.
The process eliminates common source of bias in sequencing to ensure to eliminate background noise and improve the specificity of the analysis.
Reporting Reporting

The pathogen detection results after comparative analysis will be reviewed and sorted out by medical technicians and infectious disease physicians. Finally, a report will be issued to the patient's attending physician.



   Sample collection
   Collecting sample from infected paitnets. Sample types include blood, alveolar lavage fluid, tissue, etc.

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